Spoligotyping andMycobacterium tuberculosis
نویسندگان
چکیده
منابع مشابه
Spoligotyping and Mycobacterium tuberculosis
We evaluated the clinical usefulness of spoligotyping, a polymerase chain reaction-based method for simultaneous detection and typing of Mycobacterium tuberculosis strains, with acid-fast bacilli-positive slides from clinical specimens or mycobacterial cultures. Overall sensitivity and specificity were 97% and 95% for the detection of M. tuberculosis and 98% and 96% when used with clinical spec...
متن کاملCharacterization of Finnish Mycobacterium tuberculosis isolates by spoligotyping.
The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000 to June 2001 and represented 90% of new M. tuberculosis findings by culture in the whole country during the study period. The spoligotyping results were compared to the World Spoligotyping Database...
متن کاملUse of spoligotyping for accurate classification of recurrent tuberculosis.
The spoligotyping method has become an important tool for the tracking of Mycobacterium tuberculosis strains in different epidemiological settings. In this study, we demonstrate the ability of the spoligotyping technique to accurately determine the pathogenetic mechanism of recurrent disease. This methodology has advantages over conventional restriction fragment length polymorphism methods whic...
متن کاملPulmonary tuberculosis in Harare, Zimbabwe: analysis by spoligotyping.
BACKGROUND Over the last 10 years there has been a fourfold increase in cases of tuberculosis in Harare, Zimbabwe. The use of molecular epidemiology to understand tuberculosis transmission in this epidemic has been hampered by the availability of suitable culture facilities. A study was therefore undertaken to explore the potential of spoligotyping, a polymerase chain reaction based technique t...
متن کاملMicroevolution of the direct repeat region of Mycobacterium tuberculosis: implications for interpretation of spoligotyping data.
The direct repeat (DR) region has been determined to be an important chromosomal domain for studying the evolution of Mycobacterium tuberculosis. Despite this, very little is known about microevolutionary events associated with clonal expansion and how such events influence the interpretation of both restriction fragment length polymorphism (RFLP) and spoligotype data. This study examined the s...
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ژورنال
عنوان ژورنال: Emerging Infectious Diseases
سال: 2005
ISSN: 1080-6040,1080-6059
DOI: 10.3201/eid1108.040982